Alveolar macrophage dynamics in murine lung regeneration

作者：Chamoto Kenji; Gibney Barry C; Ackermann Maximilian; Lee Grace S; Lin Miao; Konerding Moritz A; Tsuda Akira; Mentzer Steven J^*

来源：Journal of Cellular Physiology, 2012, 227(9): 3208-3215.

DOI：10.1002/jcp.24009

In most mammalian species, the removal of one lung results in dramatic compensatory growth of the remaining lung. To investigate the contribution of alveolar macrophages (AMs) to murine post-pneumonectomy lung growth, we studied bronchoalveolar lavage (BAL)-derived AM on 3, 7, 14 and 21 days after left pneumonectomy. BAL demonstrated a 3.0-fold increase in AM (CD45+, CD11b-, CD11c+, F4/80+, Gr-1-) by 14 days after pneumonectomy. Cell cycle flow cytometry of the BAL-derived cells demonstrated an increase in S?+?G2 phase cells on days 3 (11.3 +/- 2.7%) and 7 (12.1 +/- 1.8%) after pneumonectomy. Correspondingly, AM demonstrated increased expression of VEGFR1 and MHC class II between days 3 and 14 after pneumonectomy. To investigate the potential contribution of peripheral blood cells to this AM population, parabiotic mice (wild-type/GFP) underwent left pneumonectomy. Analysis of GFP+ cells in the post-pneumonectomy lung demonstrated that by day 14, less than 1% of the AM population were derived from the peripheral blood. Finally, AM gene transcription demonstrated a significant shift from decreased transcription of angiogenesis-related genes on day 3 to increased transcription on day 7 after pneumonectomy. The increased number of locally proliferating AM, combined with their growth-related gene transcription, suggests that AM actively participate in compensatory lung growth. J. Cell. Physiol. 227: 32083215, 2012.

• 出版日期2012-9