An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates

作者：Cross Trevor; Schoff Courtney; Chudoff Dylan; Graves Libby; Broomell Haley; Terry Katrina; Farina Jennifer; Correa Alexandra; Shade David; Dunbar David^*

来源：Jove-Journal of Visualized Experiments, 2015, (98): e52781.

DOI：10.3791/52781

• Environmental Sciences
• Issue 98
• Phage enrichment
• Arthrobacter phages
• phage purification
• plaque isolation
• soil phages
• Acintobacteriaceae

Bacteriophage isolation from environmental samples has been performed for decades using principles set forth by pioneers in microbiology. The isolation of phages infecting Arthrobacter hosts has been limited, perhaps due to the low success rate of many previous isolation techniques, resulting in an underrepresented group of Arthrobacter phages available for study. The enrichment technique described here, unlike many others, uses a filtered extract free of contaminating bacteria as the base for indicator bacteria growth, Arthrobactersp. KY3901, specifically. By first removing soil bacteria the target phages are not hindered by competition with native soil bacteria present in initial soil samples. This enrichment method has resulted in dozens of unique phages from several different soil types and even produced different types of phages from the same enriched soil sample isolate. The use of this procedure can be expanded to most nutrient rich aerobic media for the isolation of phages in a vast diversity of interesting host bacteria.

• 出版日期2015-4