Applicability of the Chymopapain Gene Used as Endogenous Reference Gene for Transgenic Huanong No. 1 Papaya Detection

作者：Guo, Jinchao; Yang, Litao^*; Liu, Xin; Zhang, Haibo; Qian, Bingjun; Zhang, Dabing

来源：Journal of Agricultural and Food Chemistry, 2009, 57(15): 6502-6509.

DOI：10.1021/jf900656t

• Carica papaya L.
• Chymopapain gene
• conventional PCR
• real-time quantitative PCR
• endogenous reference

The virus-resistant papaya, (Carica papaya L.), Huanong no. 1, was the genetically modified (GM) fruit approved for growing in China in 2006, To implement the labeling regulation of GM papaya and its derivates, the development of papaya endogenous reference gene is very necessary for GM papaya detection. Herein, we reported one papaya specific gene, Chymopapain (CHY), as one suitable endogenous reference gene, used for GM papaya identification. Thereafter, we established the conventional and real-time quantitative PCR assays of the CHY gene. In the CHY conventional PCR assay, the limit of detection (LOD) was 25 copies of haploid papaya genome. In the CHY real-time quantitative PCR assay, both the LOD and the limit of quantification (LOQ) were as low as 12.5 copies of haploid papaya genome. Furthermore, we revealed the construct-specific sequence of Chinese GM papaya Huanong no. 1 and developed its conventional and quantitative PCR systems employing the CHY gene as endogenous reference gene. This work is useful for papaya specific identification and GM papaya detection.

• 出版日期2009-8-12
• 单位上海交通大学