A rapid method for preparation of the cerebrospinal fluid proteome

作者：Larssen Eivind^*; Brede Cato; Hjelle Anne Bjornstad; Oysaed Kjell Birger; Tjensvoll Anne Bolette; Omdal Roald; Ruoff Peter

来源：Proteomics, 2015, 15(1): 10-15.

DOI：10.1002/pmic.201400096

• ACN-precipitation
• Cerebrospinal fluid
• LC-MS/MS
• Sample
• preparation
• Technology
• Trypsination

The cerebrospinal fluid (CSF) proteome is of great interest for investigation of diseases and conditions involving the CNS. However, the presence of high-abundance proteins (HAPs) can interfere with the detection of low-abundance proteins, potentially hindering the discovery of new biomarkers. Therefore, an assessment of the CSF subproteome composition requires depletion strategies. Existing methods are time consuming, often involving multistep protocols. Here, we present a rapid, accurate, and reproducible method for preparing the CSF proteome, which allows the identification of a high number of proteins. This method involves acetonitrile (ACN) precipitation for depleting HAPs, followed by immediate trypsination. As an example, we demonstrate that this method allows discrimination between multiple sclerosis patients and healthy subjects.

• 出版日期2015-1