New insights regarding tissue Se and Hg interactions on oxidative stress from plasma IsoP and IsoF measures in the Canadian Inuit population

作者：Alkazemi Dalal; Egeland Grace M; Roberts L Jackson II; Chan Hing M; Kubow Stan^*

来源：The Journal of Lipid Research, 2013, 54(7): 1972-1979.

DOI：10.1194/jlr.M033068

• isoprostane
• isofuran
• C-reactive protein
• mercury
• selenium

Despite animal and in vitro studies demonstrating pro-oxidative effects of Hg, previous human work showed no relationship between tissue Hg and plasma levels of F-2-isoprostanes (IsoPs), a whole-body oxidative stress marker. We hypothesized that another IsoP species, isofurans (IsoFs), was a more sensitive indicator of Hg-mediated oxidative stress, which can be modified by tissue Se status. A cross-sectional study was carried out involving individuals from a random subset (n = 233) of Inuit adults from a population-based survey (n = 2,595) of 36 Canadian Arctic Inuit communities to assess the relationships of plasma IsoPs to Se and Hg status indicators. F-2-IsoPs were inversely correlated with blood Se (r = -0.186, P = 0.005) and toenail Se (r = -0.146, P = 0.044), but not correlated with Hg. IsoFs were inversely correlated with blood Se (r = -0.164, P = 0.014) and positively correlated with Hg (r = 0.228, P %26lt; 0.001) and Hg:Se (r = 0.340, P %26lt; 0.001). The strength of the correlations remained unchanged after multivariate adjustments. Multivariate analysis showed that F-2-IsoPs were not positively associated with Hg but with Hg:Se (beta = 0.148, P = 0.021). We conclude that Se and Hg status and their interactions are important factors modulating F-2-IsoP and IsoF levels such that the Inuit may be protected from Hg-induced oxidative stress because of their high Se status.

• 出版日期2013-7