Determination of Polysaccharides in Undaria pinnatifida by Ionic Liquid-Modified Silica Gel Size Exclusion Chromatography

作者：Lee Yu Ri; Ma Wanwan; Row Kyung Ho

来源：Analytical Letters, 2018, 51(13): 1999-2012.

DOI：10.1080/00032719.2017.1413384

• Ionic liquid-modified silica
• polysaccharide
• retention factor
• size exclusion chromatography
• Undaria pinnatifida

Ionic liquid-modified silica particles with a large pore size were synthesized and used as the stationary phase for size exclusion chromatography coupled with a refractive index detector for the determination of polysaccharides, such as fucoidan, alginic acid, and laminarin from Undaria pinnatifida (seaweed). The molecular weight of polysaccharide was determined by a dextran standard curve (5-1100kDa). The ionic liquid-modified silica column exhibited excellent size exclusion properties in separating laminarin from other components. The 1-butyl-3-methyl-imidazolium bis-(trifluoromethylsulfonyl)-amino silica column has superior resolution in laminarin separation than the other columns because the amino-group in ionic liquid provide - interactions due to aromaticity of the ring structure, which enhances the effect of the hydroxyl group in the target compound separation. The concentrations of polysaccharides were calculated using a standard linear equation to be 0.332-0.484mg/g of fucoidan, 0.207-0.301mg/g of alginic acid, and 0.154-0.297mg/g of laminarin.

• 出版日期2018