Antidiarrheal effects of water-soluble proteins from Plumeria pudica latex in mice

作者：Bastos Santana Lucas de Araujo; Aragao Diego Passos; Lopes Araujo Thiago de Souza; de Sousa Nayara Alves; Miranda de Souza Luan Kelves; Silva Oliveira Lucas Eduardo; da Cunha Pereira Anna Carolina Toledo; Ferreira Gustavo Portela; de Moraes Oliveira Naylla Veras; Souza Bruna da Silva; Melo Sousa Francisca Beatriz; Ramos Marcio Viana; Teixeira de Freitas Cleverson Diniz; Rolim Medeiros Jand Venes; de Oliveira Jefferson Soares^*

来源：Biomedicine & Pharmacotherapy, 2018, 97: 1147-1154.

DOI：10.1016/j.biopha.2017.11.019

• Castor oil
• PGE(2)
• Cholera
• Proteinase
• Chitinases
• Proteinase inhibitor

The water-soluble protein fraction obtained from Plumeria pudica (LPPp) latex has previously been demonstrated to have anti-inflammatory and antinociceptive effects. In the present study, LPPp was tested for activity against diarrhea induced by castor oil, prostaglandin E-2 (PGE(2)) or cholera toxin. Different doses of LPPp (10, 20 or 40 mg/kg) significantly inhibited the percentage of diarrheal stools (31.18%, 42.97% and 59.70%, respectively) induced by castor oil. This event was followed by significant reduction of both intestinal fluid accumulation (31.42%; LPPp 40 mg/kg) and intestinal transit (68.4%; LPPp 40 mg/kg). The pretreatment of animals with LPPp (40 mg/kg) prevented glutathione and malondialdehyde alterations induced by castor oil. The effects of LPPp against diarrhea induced by castor oil were lost when the fraction was submitted to protein denaturing treatment with heat. LPPp (40 mg/kg) also inhibited the average volume of intestinal fluid induced by PGE2 (inhibition of 46.0%). Furthermore, LPPp (40 mg/kg) prevented intestinal fluid secretion accumulation (37.7%) and chloride ion concentration (50.2%) induced by cholera toxin. In parallel, colorimetric assays demonstrated that proteinases, chitinases and proteinase inhibitors were found in LPPp. Our data suggest that the antidiarrheal effect of LPPp is due to its protein content and is probably associated with its anti-inflammatory properties.

• 出版日期2018-1