Engineering of a O-6-alkylguanine-DNA alkyltransferase chimera and repair of O-4-alkyl thymidine adducts and O-6-alkylene-2 %26apos;-deoxyguanosine cross-linked DNA

作者：McManus Francis P^*; Wilds Christopher J

来源：Toxicology Research, 2013, 2(3): 158-162.

DOI：10.1039/c2tx20075a

A soluble human O-6-alkylguanine-DNA alkyltransferase (hAGT) chimera was engineered containing the active site of OGT (residues 139-159) and an additional S134P mutation. The resulting hAGT chimera not only retained hAGT%26apos;s ability to repair bulky O-6-alkylene-2%26apos;-deoxyguanosine interstrand cross-linked DNA damage but also displayed enhanced repair of various O-4-alkyl thymidine adducts.

• 出版日期2013