Schistosoma haematobium: Identification of new estrogenic molecules with estradiol antagonistic activity and ability to inactivate estrogen receptor in mammalian cells

作者：Botelho Monica Catarina^*; Soares Raquel; Vale Nuno; Ribeiro Ricardo; Camilo Vania; Almeida Raquel; Medeiros Rui; Gomes Paula; Machado Jose Carlos; Correia da Costa Jose Manuel

来源：Experimental Parasitology, 2010, 126(4): 526-535.

DOI：10.1016/j.exppara.2010.06.012

• Schistosoma haematobium
• Estradiol
• Estrogen receptor
• MCF-7 cells
• CHO cells
• Estrogenic molecules
• Mass spectrometry

We have previously identified the expression of an estradiol (E2)-related molecule by Schistosoma haematobium total antigen (Sh). We now show that this molecule has an antagonistic effect of estradiol in vitro. Our results are consistent with the existence of an estrogenic molecule that antagonizes the activity of estradiol. We found evidence for this molecule as we identified and characterized by mass spectrometry new estrogenic molecules previously unknown, present in schistosome worm extracts and sera of Schistosoma-infected individuals. We also show that Sh is able to interact in vitro with estrogen receptor (ER), explaining how host endocrine system can favor the establishment of schistosomes. These findings highlight the exploitation of the host endocrine system by schistosomes and represent an additional regulatory component of schistosome development that defines a novel paradigm enabling host-parasite interactions. The identification of these molecules opens new ways for the development of alternative drugs to treat schistosomiasis.

• 出版日期2010-12