Multi-channel microfluidic devices combined with electrospray ionization quadrupole time-of-flight mass spectrometry applied to the monitoring of glutamate release from neuronal cells

作者：Wei Huibin; Li Haifang; Gao Dan; Lin Jin Ming^*

来源：Analyst, 2010, 135(8): 2043-2050.

DOI：10.1039/c0an00162g

This paper describes an integrated system combining microfluidic devices with electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-Q-TOF-MS) for monitoring cellular chemical release. To demonstrate the feasibility of this new system, the reported carnosine-protection process against A beta 42-induced glutamate released from PC12 cells, was monitored. Poly-L-lysine coated microchannels were used to culture cells. A multi-channel miniature extraction chip (MEC) was integrated into the design to remove salts and protein interference effects. ESI-Q-TOF-MS was employed to realize semi-quantitative and highly sensitive qualitative analysis. The protective effect of carnosine against A beta 42-induced neurotoxicity was evaluated under different conditions in microchannels in parallel. The secretion product analysis, carried out by ESI-Q-TOF-MS, was accomplished in 5 min using only 2.5 mu L of solvent. Furthermore, we show that integrated microfluidic devices have significant potential for the analysis of cellular secretions, as well as for medical screening tests and for the diagnosis of specific diseases.

• 出版日期2010
• 单位清华大学