A GGCT fluorogenic probe: design, synthesis and application to cancer-related cells

作者：Yoshiya Taku^*; Ii Hiromi; Tsuda Shugo; Kageyama Susumu; Yoshiki Tatsuhiro; Nishiuchi Yuji

来源：Organic and Biomolecular Chemistry, 2015, 13(11): 3182-3185.

DOI：10.1039/c5ob00086f

Cancer-related gamma-glutamyl cyclotransferase (GGCT) specifically converts.-glutamyl amino acids (gamma-Glu-Xaa) into pyroglutamate and the corresponding amino acids (Xaa). Here we report a novel GGCT fluorogenic probe "LISA-101" containing a masked O-acylated fluorophore "resorufin" on the side chain of the P-1' amino acid (Xaa). Upon GGCT treatment, the P-1' amino acid was liberated and spontaneously released the intact fluorophore. Thus, the fluorescence was regained. LISA-101 will expand the strategies for cancer studies.

• 出版日期2015