Transplantation of Neural Stem Cells Overexpressing Cardiotrophin-1 Inhibits Sprouting of Hippocampal Mossy Fiber in a Rat Model of Status Epilepticus

作者：Shu, Xiaomei^*; Du, Shuzhen; Chen, Xuemei; Li, Zhenhong

来源：Cell Biochemistry and Biophysics, 2011, 61(2): 367-370.

DOI：10.1007/s12013-011-9219-z

• Status epilepticus
• Cardiotrophin 1
• Neural stem cells
• Transplantation
• Mossy fiber sprouting

In this study, we studied the effects of hippocampal transplantation of neural stem cells (NSCs) overexpressing cardiotrophin 1 (CT1) on hippocampal mossy fiber sprouting (MFS) in a rat model of status epilepticus (SE). SE rats (lithium-pilocarpine model) were randomized into four study groups (18 rats per group): CT1-NSCs group, NSCs group, SE control group, and normal control group. Six rats were randomly chosen from each group at 1, 4, and 8 weeks after transplantation. MFS in hippocampal dentate gyrus was scored (Timm staining) at these time points. The MFS scores were as follows: CT1-NSCs 0.77 +/- A 0.04, 2.48 +/- A 0.89, and 2.39 +/- A 0.82 (1, 4, and 8 weeks after transplantation, respectively); NSCs 1.12 +/- A 0.62, 3.17 +/- A 0.64, and 3.88 +/- A 0.51; SE control 1.32 +/- A 0.35, 3.28 +/- A 0.75, and 4.32 +/- A 1.55; and normal control 0.37 +/- A 0.06, 0.34 +/- A 0.07, and 0.43 +/- A 0.04. Compared to SE control group and NSCs group, the scores of MFS in CT1-NSCs group were significantly lower (P < 0.05). In conclusion, transplantation with NSCs overexpressing CT1 inhibits hippocampal MFS and facilitates reduction of recurrent seizures.

• 出版日期2011-11
• 单位遵义医科大学