A NOVEL MUTATION OCCURRING IN THE PHO80 GENE SUPPRESSES THE PHO4C MUTATIONS OF SACCHAROMYCES-CEREVISIAE

作者：OKADA H; TOHE A

来源：Current Genetics, 1992, 21(2): 95-99.

DOI：10.1007/BF00318466

• PHO
• SACCHAROMYCES
• PROTEIN-PROTEIN INTERACTION
• REGULATION

We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isoleucine for methionine 42 of the Pho80 protein. Substitution of valine (PHO80-3) or leucine (PHO80-4) for the same methionine by site-directe

• 出版日期1992-2