Differentiation of fetal pancreatic stem cells into neuron-like and islet-like cells in vitro

作者：Hua, Xiufeng^*; Wang, Yanwei; Lian, Peiwen; Zhang, Shouxin; Li, Jianyuan; Wang, Haiyan; Chen, Shulin; Gao, Wei

来源：Neural Regeneration Research, 2012, 7(7): 506-510.

DOI：10.3969/j.issn.1673-5374.2012.07.005

• fetal pancreas
• pancreatic stem cells
• differentiation
• islet-like cells
• neuron-like cells
• neural regeneration

Pancreatic stem cells were isolated and cultured from aborted human fetal pancreases of gestational age 14-20 weeks. They were seeded at a density of 1 x 10(4) in serum-free media for differentiation into neuron-like cells, expressing beta-tubulin III and glial fibrillary acidic protein. These neuron-like cells displayed a synapse-like morphology and appeared to form a neuronal network. Pancreatic stem cells were also seeded at a density of 1 x 10(5) for differentiation into islet-like cells, expressing insulin and glucagon, with an islet-like morphology. These cells had glucose-stimulated secretion of human insulin and C-peptide. Results suggest that pancreatic stem cells can be differentiated into neuron-like and islet-like cells.

• 出版日期2012-3-5
• 单位烟台毓璜顶医院