Dietary essential alpha-linolenic acid and linoleic acid differentially modulate TNF alpha-induced NF kappa B activity in FADS2-deficient HEK-293 cells

作者：Schuebel Ruth; Jaudszus Anke; Krueger Ralf; Roth Alexander; Klempt Martin; Barth Stephan Wilhelm^*

来源：International Journal of Food Sciences and Nutrition, 2017, 68(5): 553-559.

DOI：10.1080/09637486.2016.1265918

• Delta-6 desaturase
• fatty acid desaturase
• inflammation
• polyunsaturated fatty acids

The pro- or anti-inflammatory bioactivity of dietary essential linoleic acid (LA) and alpha-linolenic acid (ALA) is mainly attributed to rate-limiting delta-6 desaturase (D6D) activity. The aim of this study was to analyze mechanisms of D6D-substrates ALA, LA and D6D-product gamma-linolenic acid (GLA) under D6D-deficient conditions. Fatty acid profiles (GC-MS), D6D gene expression (real-time RT-PCR) and NFB activity (luciferase assay) were assessed in HEK293 cells. FADS2 gene expression was approved being marginal. Incubation with ALA or LA did not increase D6D products but their elongase products C20:3n-3 and C20:2n-6. Bypassing the D6D, GLA elevated C20:3n-6 and C20:4n-6. LA significantly increased (+18% at 60M; p<.001), ALA reduced (-32% at 100M; p<.001) and GLA did not specifically change NFB activity. Our data indicate that D6D might not be essential for the distinct effects of LA and ALA on NFB activity.

• 出版日期2017