Anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea Javanica

作者：Yang, Jianhong; Li, Shucai; Xie, Caifeng; Ye, Haoyu; Tang, Huan; Chen, Lijuan^*; Peng, Aihua

来源：Journal of Ethnopharmacology, 2013, 147(2): 442-446.

DOI：10.1016/j.jep.2013.03.034

• Brucea javanica
• Anti-inflammatory
• Pro-inflammatory mediators
• Carrageenan induced paw edema test

Ethnopharmacological relevance: The seeds of Brucea javanica (L) Men. (Yadanzi in Chinese) have been used for the treatment of inflammation, dysentery, malaria, and cancer in Chinese traditional medicine. However, the anti-inflammatory mechanism of Brucea javanica has not been fully elucidated. This study examined the anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea javanica (EA-BJ) in vitro and in vivo.
Materials and methods: The anti-inflammatory activity of EA-BJ and its ability to modulate the production of NO, PGE2, TNF-alpha, IL-1 beta, IL-6 and IL-10 inflammatory mediators in lipopolysaccharide-activated RAW 264.7 macrophage were evaluated. Moreover, the anti-inflammatory activity of EA-BJ was also in vivo assayed by carrageenan induced paw edema in mice.
Results: In vitro assays showed remarkable anti-inflammatory activity of EA-BJ, through the inhibition of production of NO, PGE2, TNF-alpha, IL-1 beta and IL-6 inflammatory mediators and induction of production of IL-10 anti-inflammatory cytokine. In vivo assays showed anti-inflammatory activity for decrement of the paw edema in carrageenan induced paw edema test.
Conclusion: The results obtained in vitro and in vivo showed that possible anti-inflammatory effects of EA-BJ may be attributed to inhibition pro-inflammatory mediators production, NO, PGE2, TNF-alpha, IL-1 beta and IL-6 and to increase production of IL-10 anti-inflammatory cytokine. The seeds of Brucea javanica may thus prove beneficial in the treatment of inflammatory diseases.

• 出版日期2013-5-20
• 单位生物治疗国家重点实验室; 四川大学