Assessment of IL-6, IL-8 and TNF-alpha levels in the gingival tissue of patients with periodontitis

作者：Noh Min Ki; Jung Min; Kim Soo Hwan; Lee Seo Ra; Park Ki Ho; Kim Dong Hwan; Kim Hanna Hyun; Park Young Guk^*

来源：Experimental and Therapeutic Medicine, 2013, 6(3): 847-851.

DOI：10.3892/etm.2013.1222

• interleukin-6
• interleukin-8
• tumor necrosis factor-alpha
• periodontitis

In periodontal diseases, inflammatory mediators, including interleukin (IL)-6, IL-8 and tumor necrosis factor-alpha (TNF-alpha), may promote the degeneration of inflamed periodontal tissues. In previous studies, levels of these three cytokines were demonstrated to be elevated in inflammatory gingival tissues and gingival crevicular fluid. The aim of the present study was to quantify IL-6, IL-8 and TNF-alpha levels in the human gingival tissues of patients with periodontitis and to assess the correlation of these three cytokines with each other. In this study, human gingival tissues from 19 patients with periodontitis (male, n=14; female, n=5) were collected. The tissues were homogenized, centrifuged and the protein in the supernatant was quantified. Enzyme-linked immunosorbent assay (ELISA) was used in the measurement of the IL-6, IL-8 and TNF-alpha levels, and the mean levels were observed to be 8.41 +/- 0.25, 34.01 +/- 1.09 and 20.70 +/- 0.31 pg/ml, respectively. The mean levels of IL-8 were higher than those of the other two cytokines. In each sample, the level of TNF-alpha expression was consistently high, with little difference between the results, which contrasted with the fluctuations in IL-6 and IL-8 levels. The expression of the two ILs (IL-6 and IL-8) showed a positive correlation (r=0.932, P=0.01), whereas TNF-alpha levels were not correlated with IL-6 or IL-8 levels. These results suggest that IL-6, IL-8 and TNF-alpha may be relevant in the pathophysiology of periodontitis, and the measurement of these cytokines may be beneficial in the identification of patients with periodontitis.

• 出版日期2013-9