Molecular species identification of commercially important penaeid shrimp from the Gulf of Mexico using a multiplex haplotype-specific PCR assay

作者：Bremer Jaime R Alvaiado^*; Ditty James G; Turner Jennifer S; Saxton Brandon L

来源：Biochemical Systematics and Ecology, 2010, 38(4): 715-721.

DOI：10.1016/j.bse.2010.05.006

• Multiplex PCR
• Penneid shrimp
• Species identification
• Farfantepenaeus aztecus
• F brasiliensis
• F duorarum
• Litopenaeus vannamei
• L setiferus
• Brown shrimp
• Pinkspot shrimp
• Pink shrimp
• Northern white shrimp
• Pacific white shrimp

This study describes a multiplex PCR assay based on the 16S rRNA mitochondrial gene to identify the penaeid shrimp Farfantepenaeus aztecus Farfantepenaeus duorarum Farfante penaeus brasthensis and Litopenaeus setiferus all native to the Gulf of Mexico and the exotic Litopenaeus vannamei The assay was validated using positively identified adult shrimp and confirmed by direct sequencing Samples of postlarvae and early juveniles collected in the eastern and western Gulf of Mexico were tested yielding 119 F aztecus 78 F duorarum and five L setiferus Reliable identification of the morphologically similar early life stages of F azterus and F duorarum has important implications for management and conserva

• 出版日期2010-8