MicroRNA profiling of rats with ochratoxin A nephrotoxicity

作者：Dai Qiu; Zhao Jue; Qi Xiaozhe; Xu Wentao^*; He Xiaoyun; Guo Mingzhang; Dweep Harsh; Cheng Wen Hsing; Luo Yunbo; Xia Kai; Gretz Norbert; Huang Kunlun

来源：BMC Genomics, 2014, 15(1): 333.

DOI：10.1186/1471-2164-15-333

• High throughput sequencing
• Ochratoxin A
• miRNA biogenesis
• miRNA expression
• Nephrotoxicity

Background: Nephrotoxicity is the most prominent one among the various toxicities of ochratoxin A (OTA). MicroRNAs (miRNAs) are small non-coding RNAs that have an impact on a wide range of biological processes by regulating gene expression at post-transcriptional level or protein systhesis level. The objective of this study is to analyze miRNA profiling in the kidneys of rats gavaged with OTA. %26lt;br%26gt;Results: To profile miRNAs in the kidneys of rats with OTA nephrotoxicity, high-throughput sequencing and bioinformatics approaches were applied to analyze the miRNAs in the kidney of rats following OTA treatment. A total of 409 known miRNAs and 8 novel miRNAs were identified in the kidney and the levels of the novel miRNAs were varied in response to different doses of OTA. Expression of miR-129, miR-130a, miR-130b, miR-141, miR-218b and miR-3588 were uniquely suppressed in mid dose but then elevated in high dose, with opposite expression to their target genes. The expression pattern was closely related with the %26quot;MAPK signaling pathway%26quot;. Dicer1 and Drosha were significantly suppressed, indicating an impairment of miRNA biogenesis in response to OTA. %26lt;br%26gt;Conclusions: The abrogation of miRNA maturation process suggests a new target of OTA toxicity. Moreover, the identification of the differentially expressed miRNAs provides us a molecular insight into the nephrotoxicity of OTA.

• 出版日期2014-5-5
• 单位中国农业大学