A fully automatic method yielding initial models from high-resolution cryo-electron microscopy maps

作者：Terwilliger, Thomas C.^*; Adams, Paul D.; Afonine, Pavel V.; Sobolev, Oleg V.

来源：Nature Methods, 2018, 15(11): 905-+.

DOI：10.1038/s41592-018-0173-1

• CRYO-EM STRUCTURE
• SEQUENCE ASSIGNMENT
• REFINEMENT
• COMPLEX
• SYSTEM
• PHENIX

We report a fully automated procedure for the optimization and interpretation of reconstructions from cryo-electron microscopy (cryo-EM) data, available in Phenix as phenix. map_to_model. We applied our approach to 476 datasets with resolution of 4.5 angstrom or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 angstrom or better and 47% for those at resolutions worse than 3 angstrom.

• 出版日期2018-11
• 单位上海大学; Los Alamos