Nanoemulsification Potentiates In Vivo Antiedematogenic Effect of Copaiba Oil

作者：Lucca Letcia G; de Matos Sheila P; de Mattos Cristiane B; Teixeira Helder F; Limberger Renata P; Veiga Valdir F Jr; de Araujo Bibiana V; Koester Leticia S

来源：Journal of Biomedical Nanotechnology, 2017, 13(5): 583-590.

DOI：10.1166/jbn.2017.2366

• Copaifera multijuga
• Skin Permeation
• beta-Caryophyllene
• Rat Paw Edema
• Mouse Ear Edema

Copaiba oil is a natural product obtained from the trunk of Copaifera trees. This oil-resin is used in folk medicine in Amazonia as an anti-inflammatory, antiparasitary and antimicrobial. beta-caryophyllene, a major component in copaiba oil, had its anti-inflammatory effect studied in recent papers and is used as a copaiba biomarker. In the present study, we developed positively charged copaiba oil nanoemulsions (PCN), with cetyltrimethylammonium bromide and oleylamine, and compared to a negatively charged nanoemulsion (NCN) concerning skin permeation and in vivo antiedematogenic effect. Results show that skin permeation with the PCN increased three fold beta-caryophyllene retention in the epidermis, and also in the receptor fluid compared to the NCN. In vivo tests were performed in mouse ear edema induced by arachidonic acid and in rat paw edema induced by formalin. In mouse ear edema, NCN and PCN promoted an edema inhibition (33%) with statistically equal effect (p > 0.05) to the positive control, ketoprofen (44%). In rat paw edema, both nanoemulsions presented antiedematogenic effect (edema inhibition above 60%) similar to the positive control. Copaiba oil also exhibited edema inhibition, but the nanoemulsification process led to an increased effect to the oil.

• 出版日期2017-5