Ultrastructure of Vascular Permeability in Urticaria

作者：Criado Paulo R^*; Criado Roberta F J; Takakura Cleusa F H; Pagliari Carla; de Carvalho Jozelio F; Sotto Mirian N; Vasconcellos Cidia

来源：Israel Medical Association Journal, 2013, 15(4): 239-243.

• urticaria
• drug adverse reaction
• ultrastructure
• vascular permeability
• caveola

Background: Few studies have addressed the ultrastructure of vascular permeability in urticaria.
Objectives: To describe the types of endothelial cell organelles involved in vascular permeability in drug induced acute urticaria (DIAU).
Methods: Seven patients with DIAU were enrolled in the study. Biopsies of urticarial lesions and apparently normal skin were performed. The 14 collected fragments were processed with immunogold electron microscopy using single stains for tryptase and factor XIIIa (FXIIIa) and double immunogold labeling for both tryptase and FXIIIa.
Results: Some sections demonstrated mast cells in the degranulation process, in both anaphylactic and piecemeal degranulation. After double immunogold staining, 10 nm (FXIIIa) and 15 nm (tryptase) gold particles were both present, covering the granules in the mast cells, indicating that both tryptase and FXIIIa were localized within the granules of these cells. Interestingly, we found strong evidence of the presence of caveolae and vesico-vacuolar organelles (VVOs) in the endothelial cells of the biopsies. In addition to these findings, we were able to demonstrate the presence of tryptase and FXIIIa in the endothelial cells, in urticarial lesions and in apparently normal skin.
Conclusions: VVOs are present in the endothelial cells of post capillary venules in DIAU. This is the first report on the expression of FXIIIa and tryptase in the cytoplasm of endothelial cells in urticaria.

• 出版日期2013-4