Association between UDP-glucuronosyltransferase 2B7 tagSNPs and breast cancer risk in Chinese females

作者：He, Bao-Xia^*; Qiao, Bin; Lam, Alfred King-Yin; Zhao, Xiu-Li; Zhang, Wen-Zhou; Liu, Hui

来源：CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2018, 45(5): 437-443.

DOI：10.1111/1440-1681.12908

• breast cancer
• polymorphism
• sex hormones
• susceptibility
• UDP-glucuronosyltransferase (UGT) 2B7

This retrospective study was performed to evaluate the association between the UGT2B7 tagSNPs (rs12233719, rs4356975, rs7435335 and rs7441774) and breast cancer in Chinese females. Blood samples were collected from 672 patients with breast cancer and 670 healthy controls for DNA extraction. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to analyze UGT2B7 polymorphisms. Dual-luciferase reporter assays were further performed to investigate the regulatory function of UGT2B7 tagSNPs. The frequency of rs7441774 G allele in the breast cancer cases was statistically significantly higher than in the controls (0.412 vs 0.358, P=.006; odds ratio [OR]=1.27, 95% CI=1.08-1.48). After adjusting for conventional risk factors, individuals with the GG genotype had a higher breast cancer risk than those with the AA genotype (adjusted OR=1.63, 95% CI=1.18-2.26; P=.008). The GCGG haplotype of UGT2B7 was also associated with breast cancer (OR=1.22, 95% CI=1.04-1.45; P=.027). Meanwhile, the rs7441774 G allele could significantly decrease the transcriptional activity of the UGT2B7 gene. This study indicates that UGT2B7 polymorphisms may play a crucial role in the occurrence and development of breast cancer in the Han Chinese population.

• 出版日期2018-5
• 单位郑州大学