A simple method of labeling amyloid beta with quantum dots and ingestion of the labeled amyloid beta by astrocytes

作者：Zhang Jing^*; Jia Xing; Qing Hong; Xie Hai Yan

来源：Nanotechnology, 2013, 24(3): 035101.

DOI：10.1088/0957-4484/24/3/035101

Steady labeling of amyloid beta (A beta)is crucial for studying the ingestion and degradation of A beta by astrocytes and unraveling a relevant regulation mechanism. Quantum dots (QDs) are an optimum labeling reagent for this because of their strong and steady fluorescence properties. In this paper, A beta was labeled with QDs by a simple mixed incubation strategy, with a QD labeled A beta complex (QDs-A beta) being obtained. In the complex, QDs efficiently restrained the formation of beta-folding and fibrils of A beta, while the graininess, dispersivity and fluorescence properties of the QDs hardly changed. The fluorescence microscopy imaging results showed that the astrocytes could ingest the QDs-A beta. The QDs and A beta did not separate from each other during the ingestion process, and the A beta could be degraded subsequently.

• 出版日期2013-1-25
• 单位北京理工大学