Attenuation of nociceptive pain and inflammatory disorders by total steroid and terpenoid fraction of Euphorbia tirucalli Linn root in experimental in vitro and in vivo model

作者：Palit Partha^*; Mukherjee Dhrubojyoti; Mahanta Poulami; Shadab Md; Ali Nahid; Roychoudhury Shubhadeep; Asad Md.; Mandal Subhash C.

来源：InflammoPharmacology, 2018, 26(1): 235-250.

DOI：10.1007/s10787-017-0403-7

• Euphorbia tirucalli
• Nociceptive pain
• Anti-inflammatory
• Anti-arthritic effect
• Anti-asthmatic
• Cytokine
• Cyclooxygenase
• Inducible nitric-oxide synthase

The plant Euphorbia tirucalli Linn has been successfully used as a tribal folk medicine in India and Africa for the management of acute inflammatory, arthritic, nociceptive pain and asthmatic symptoms. The present study was conducted to assess the anti-inflammatory, analgesic, anti-asthmatic and anti-arthritic role of the total steroid and terpenoid rich fractions of the hydro-alcoholic extract of E. tirucalli root (STF-HAETR). STF-HAETR fraction demonstrated 71.25 +/- 2.5 and 74.25 +/- 5.1% protection against acetic acid-induced pain and central neuropathic pain at 75 and 100 mg/kg doses, respectively. It showed 96.97% protection against acute inflammation at 100 mg/kg with 1.6-fold better activity than the standard drug. The fraction exhibited such efficacy via inhibition of proinflammatory cytokines TNF-alpha, IFN-gamma, by 61.12 and 65.18%, respectively, at 100 mu g/mL. Inhibition of cyclooxygenase and Nitric oxide synthase in a dose-dependent manner affirms its analgesic and anti-inflammatory activity. The spectrophotometric analysis reveals that STF-HAETR induces ameliorative effect against heat-induced denaturation of Bovine serum albumin (BSA) and exhibits significant anti-proteinase activity. The plant fraction also demonstrated anti-asthmatic activity by displaying 62.45% protection against histamine induced bronchoconstriction or dyspnoea. Our findings suggest that STF-HAETR could be an effective safe therapeutic agent to treat nociceptive pain, acute inflammation, asthma, and arthritis which may authenticate its traditional use.

• 出版日期2018-2