Upregulation of Pro-inflammatory Cytokine Expression Following Chronic Paracetamol Treatment in Astrocyte

作者：Tantarungsee Nutnicha; Yisarakun Waranurin; Thongtan Thananya; Lalert Laddawan; Srikam Sirinapa; Reuangwechvorachai Preecha; Ingruanglert Praewphan; Maneesri le Grand Supang^*

来源：Neurotoxicity Research, 2018, 34(1): 137-146.

DOI：10.1007/s12640-018-9875-5

• Paracetamol
• Astrocyte
• Pro-inflammatory cytokines
• NF-kB signaling
• Ultrastructure

The present study aimed to investigate the effect of APAP treatment on the expression of pro-inflammatory cytokines in the astrocytes. The mouse astrocyte cells (C8-D1A) were treated with APAP at the concentration of 100 mu M for 24 h, 16 and 28 days. The expressions of pro-inflammatory cytokines and NF-kB were determined using western blot analysis. Furthermore, the expression and localization of phosphorylation of NF-kB were detected by immunohistochemical and immunofluorescent analysis. The ultrastructure of C8-D1A cells was as well monitored. The results revealed that acute APAP treatment (24 h) had no effect on the expression of pro-inflammatory cytokines and pNF-kB. This treatment did not alter the ultrastructure of C8-D1A cells when compared with those in the control cells. However, the results obtained from the study on chronic APAP-treated cells (16 and 28 days) showed the different effect of APAP treatment. The results obtained from western blot analysis showed the increment of pro-inflammatory cytokine (IL-1 beta and TNF-alpha) expressions and the activation of NF-kB signaling pathway. Nuclear translocation of pNF-kB and alteration of several cell structures were well observed in the C8-D1A cells with chronic APAP treatment. The results obtained from this study suggest that chronic APAP treatment can induce an upregulation of pro-inflammatory cytokines (IL-1 beta and TNF alpha) in astrocytes. This alteration implies the involvement of the activation of NF-kB signaling pathway.

• 出版日期2018-7