Development, characterisation and cross-species amplification of 16 novel microsatellite markers for the endangered Black-throated Finch (Poephila cincta) in Australia

作者：Tang Lei^*; Smith Keune Carolyn; Gardner Michael G; Hardesty Britta Denise

来源：Conservation Genetics Resources, 2014, 6(1): 143-146.

DOI：10.1007/s12686-013-0028-0

• Black-throated Finch
• Poephila cincta
• Microsatellite
• Conservation genetics
• Next generation sequencing

The Black-throated Finch (Southern) (Poephila cincta cincta) is threatened by the substantial landscape changes in northern Australia. We developed 16 polymorphic microsatellite markers using 454-shotgun whole-genome sequencing technology. We identified an average of 4.7 alleles per locus based on 63 wild caught individuals from Townsville, Queensland. Thirteen and 9 markers were also successfully cross-amplified in two confamilial species, the Double-barred Finch (Taeniopygia bichenovii) and the chestnut-breasted Mannikin (Lonchura castaneothorax) with 11 and 5 were polymorphic, respectively. These markers will help understand the population genetic structure of the endangered Black-throated Finch and determine genetic consequences of landscape changes for the species.

• 出版日期2014-3
• 单位CSIRO