Disrupting assembly of the inner membrane complex blocks Plasmodium falciparum sexual stage development

作者：Schneider Molly Parkyn; Liu Boyin; Glock Philipp; Suttie Annika; McHugh Emma; Andrew Dean; Batinovic Steven; Williamson Nicholas; Hanssen Eric; McMillan Paul; Hliscs Marion; Tilley Leann; Dixon Matthew W A^*

来源：PLoS Pathogens, 2017, 13(10): e1006659.

DOI：10.1371/journal.ppat.1006659

Transmission of malaria parasites relies on the formation of a specialized blood form called the gametocyte. Gametocytes of the human pathogen, Plasmodium falciparum, adopt a crescent shape. Their dramatic morphogenesis is driven by the assembly of a network of microtubules and an underpinning inner membrane complex (IMC). Using super-resolution optical and electron microscopies we define the ultrastructure of the IMC at different stages of gametocyte development. We characterize two new proteins of the gametocyte IMC, called PhIL1 and PIP1. Genetic disruption of PhIL1 or PIP1 ablates elongation and prevents formation of transmission-ready mature gametocytes. The maturation defect is accompanied by failure to form an enveloping IMC and a marked swelling of the digestive vacuole, suggesting PhIL1 and PIP1 are required for correct membrane trafficking. Using immuno-precipitation and mass spectrometry we reveal that PhIL1 interacts with known and new components of the gametocyte IMC.

• 出版日期2017-10