Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype, allozyme and RAPD analyses

作者：You Feng^*; Wang Wei; Xu Dongdong; Zhu Xiangping; Ni Jing; Wu Zhihao; Xu Yongli; Wang Xincheng; Zhang Peijun

来源：Chinese Journal of Oceanology and Limnology, 2009, 27(2): 317-323.

DOI：10.1007/s00343-009-9134-y

• artificial hybridization
• Paralichthys olivaceus (Temminck et Schlegel)
• Kareius bicoloratus (Basilewsky)
• karyotype
• allozyme
• RAPD

The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish.

• 出版日期2009-6
• 单位青岛农业大学; 中国科学院海洋研究所