Determination of scutellarin in mouse plasma and different tissues by high-performance liquid chromatography

作者：Xiong F; Wang H; Cheng J; Zhu JB^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2006, 835(1-2): 114-118.

DOI：10.1016/j.jchromb.2006.02.041

• scutellarin

A simple HPLC-UV method was developed for the determination of scutellarin in plasma and different tissues of mice (heart, liver, spleen, lungs and kidneys). The separation was achieved by HPLC on a Hypersil C-18 column with a mobile phase composed of methanol-water-glacial acetic acid (40:60: 1). UV detection was used at 335 nm. The calibration curves were linear in all matrices (r(2) > 0.997) in the concentration range of 0.1-10 mu g/ml for plasma and 0.1-20 mu g/g for tissue homogenates, respectively. The method described is suitable for studies on the distribution of scutellarin in different tissues of mice.

• 出版日期2006-5-1
• 单位中国药科大学