Alternative splicing and expression analysis of High expression of osmotically responsive genes1 (HOS1) in Arabidopsis

作者：Lee Jeong Hwan; Kim Soo Hyun; Kim Jae Joon; Ahn Ji Hoon^*

来源：BMB Reports, 2012, 45(9): 515-520.

DOI：10.5483/BMBRep.2012.45.9.092

• AAB87130
• Alternative splicing
• BT005517
• HOS1
• Post-transcriptional regulation

High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time, encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L) of HOS1. Comparative analyses revealed that HOS1-L has a longer 5%26apos; nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that its protein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1 expression at the post-transcriptional level. [BMB Reports 2012; 45(9): 515-520]

• 出版日期2012-9-30