Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale, 1905 (Clupeiformes: Engraulidae) and cross-species amplification in Coilia mystus Linnaeus, 1758

作者：Ma, Chunyan; Cheng, Qiqun^*; Zhang, Qingyi

来源：Environmental Biology of Fishes, 2011, 91(2): 243-249.

DOI：10.1007/s10641-011-9766-6

• Coilia ectenes
• Coilia mystus
• Coilia nasus
• Microsatellites markers
• Cross-species amplification
• Genetic structure

In this study, twelve polymorphic microsatellite markers were developed for Coilia ectenes (synonym C. nasus). In a sample of 30 C. ectenes individuals in a Wuhan population from Yangtze River, a total of 78 alleles were detected, and the number of alleles per locus ranged from 3 to 12. The observed and expected heterozygosities ranged from 0.100 to 1.000 and from 0.098 to 0.899, respectively. Six loci significantly deviated from the Hardy-Weinberg equilibrium after Bonferroni correction (P < 0.004), and no significant linkage disequilibrium between pairs of loci was found. The applicability of these markers in a closely related species, C. mystus, was evaluated by cross-species amplification. These microsatellite markers will be useful in studies on population genetics, conservation genetics, and fishery management and in the construction of genetic linkage maps of C. ectenes and C. mystus.

• 出版日期2011-6
• 单位中国水产科学研究院东海水产研究所