A novel HSP70 gene of Schizosaccharomyces pombe that confers K-252a resistance

作者：Usui T; Yoshida M; Kasahara K; Honda A; Beppu T; Horinouchi S

来源：Gene, 1997, 189(1): 43-47.

DOI：10.1016/S0378-1119(96)00831-1

• heat shock protein 70
• nucleotide sequencing
• protein kinase inhibitor
• cell cycle

A new gene encoding a heat shock protein 70 family protein of Schizosaccharomyces pombe (Sp), named sks2(+), was cloned as a weak suppressor for the K-252a-sensitive mutation, ucml. The nucleotide sequence of sks2(+) revealed an open reading frame of a 613-amino-acid (aa) protein. The deduced aa sequence of sks2(+) showed significant homology with Saccharomyces cerevisiae (Sc) Ssb1p and Ssb2p responsible for protein synthesis by non-organelle-localized ribosomes, as well as with other proteins of the HSP70 family. The cells lacking the functional sks2(+) gene were viable and showed no increased sensitivity to K-252a but grew slowly with an elongated morphology. These results suggest that the sks2(+) gene product plays a role in the cell cycle progression and is able to confer drug resistance in a multicopy state.

• 出版日期1997-4-11