Schisandrin B Prevents Hind Limb from Ischemia-Reperfusion-Induced Oxidative Stress and Inflammation via MAPK/NF-κB Pathways in Rats

作者：Zhu, Ning; Cai, Changhong; Zhou, Aiming; Zhao, Xuyong; Xiang, Yijia; Zeng, Chunlai^*

来源：Biomed Research International, 2017, 2017: 4237973.

DOI：10.1155/2017/4237973

• ACTIVATED PROTEIN-KINASE
• ISCHEMIA/REPERFUSION INJURY
• CEREBRAL-ISCHEMIA
• SKELETAL-MUSCLE
• FREE-RADICALS
• IN-VITRO
• INHIBITION
• ANTIOXIDANT

Schisandrin B (ScB), isolated fromSchisandra chinensis (S. chinensis), is a traditionalChinesemedicinewith proven cardioprotective and neuroprotective effects. However, it is unclear whether ScB also has beneficial effects on rat hind limb ischemia/reperfusion (I/R) injury model. In this study, ScB (20mg/kg, 40mg/kg, and 80mg/kg) was administered via oral gavage once daily for 5 days before the surgery. After 6 h ischemia and 24 h reperfusion of left hind limb, ScB reduced I/R induced histological changes and edema. ScB also suppressed the oxidative stress through decreasing MDA level and increasing SOD activity. Moreover, above changes were associated with downregulated TNF-alpha. mRNA expression and reduced level of IL-1 beta. in plasma. Meanwhile, ScB treatment downregulated activation of p38MAPK, ERK1/2, and NF-kappa B in ischemic skeletal muscle. These results demonstrate that ScB treatment could prevent hind limb I/R skeletal muscle injury possibly by attenuating oxidative stress and inflammation via p38MAPK, ERK1/2, and NF-kappa B pathways.

• 出版日期2017
• 单位温州医科大学