Nitrogen K-edge x-ray absorption near edge structure of pyrimidine-containing nucleotides in aqueous solution

作者：Shimada Hiroyuki^*; Minami Hirotake; Okuizumi Naoto; Sakuma Ichiro; Ukai Masatoshi; Fujii Kentaro; Yokoya Akinari; Fukuda Yoshihiro; Saitoh Yuji

来源：Journal of Chemical Physics, 2015, 142(17): 175102.

DOI：10.1063/1.4919744

X-ray absorption near edge structure (XANES) was measured at energies around the N K-edge of the pyrimidine-containing nucleotides, cytidine 5'-monophosphate (CMP), 2'-deoxythymidine 5'-monophosphate (dTMP), and uridine 5'-monophosphate (UMP), in aqueous solutions and in dried films under various pH conditions. The features of resonant excitations below the N K-edge in the XANES spectra for CMP, dTMP, and UMP changed depending on the pH of the solutions. The spectral change thus observed is systematically explained by the chemical shift of the core-levels of N atoms in the nucleobase moieties caused by structural changes due to protonation or deprotonation at different proton concentrations. This interpretation is supported by the results of theoretical calculations using density functional theory for the corresponding nucleobases in the neutral and protonated or deprotonated forms.

• 出版日期2015-5-7