科研之友
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摘要
The interaction between fullerene C60 and catalase enzyme was studied with a fullerene C60-coated piezoelectric (PZ) quartz crystal sensor. The partially irreversible response of the C60-coated PZ crystal sensor for catalase was observed by the desorption study, which implied that C60 could chemically react with catalase. Thus, immobilized fullerene C60-catalase enzyme was synthesized and applied in determining hydrogen peroxide in aqueous solutions. An oxygen electrode detector with the immobilized C60-catalase was also employed to detect oxygen, a product of the hydrolysis of hydrogen peroxide which was catalyzed by the C60-catalase. The oxygen electrode/C60-catalase detection system exhibited linear responses to the concentration of hydrogen peroxide and amount of immobilized C60-catalase enzyme that was used. The effects of pH and temperature on the activity of the immobilized C60-catalase enzyme were also investigated. Optimum pH at 7.0 and optimum temperature at 25 degrees C for activity of the insoluble immobilized C60-catalase enzyme were found. The immobilized C60-catalase enzyme could be reused with good repeatability of the activity. The lifetime of the immobilized C60-catalase enzyme was long enough with an activity of 93% after 95 days. The immobilized C60-catalase enzyme was also applied in determining glucose which was oxidized with glucose oxidase resulting in producing hydrogen peroxide, followed by detecting hydrogen peroxide with the oxygen electrode/C60-catalase detection system.
- 出版日期2009-8
