A novel three-fluorophore system as a ratiometric sensor for multiple protease detection

作者:Okorochenkova Yana; Porubsky Martin; Benicka Sandra; Hlavac Jan*
来源:Chemical Communications, 2018, 54(55): 7589-7592.
DOI:10.1039/c8cc01731j

摘要

A synthetic three-fluorophore system with two enzymatically cleavable linkers has been developed for the simultaneous detection of two proteases in a mixture. The probe was designed to afford single excitation/triple emission ratiometric detection through a fluorescence change during the cleavage of a peptide linker. The developed assays were verified for trypsin and chymotrypsin as the model enzymes.

  • 出版日期2018-7-14