Design and evaluation of an electrochemical immunosensor for measles serodiagnosis using measles-specific Immunoglobulin G antibodies

作者：Mashazi Philani^*; Vilakazi Sibulelo; Nyokong Tebello

来源：Talanta, 2013, 115: 694-701.

DOI：10.1016/j.talanta.2013.06.036

• Measles-antigen
• Measles-specific antibodies
• Horse-radish peroxidase
• Labeled-detection
• Immunosensors

The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL(-1) with R-2=0.997 and the limit of detection of 6.60 ng mL(-1) at 3 sigma. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples.

• 出版日期2013-10-15