Melanocortin-4 receptor (MC4R) polymorphisms are associated with growth and meat quality traits in sheep

作者：Zuo, Beiyao; Liu, Guiqiong^*; Peng, Yuqin; Qian, Hongguang; Liu, Jiasen; Jiang, Xunping; Mara, Adama

来源：Molecular Biology Reports, 2014, 41(10): 6967-6974.

DOI：10.1007/s11033-014-3583-x

• Melanocortin 4 receptor gene
• Sheep
• Single nucleotide polymorphisms
• Body weight
• Loin-eye area
• Average daily gain

The involvement of melanocortin 4 receptor gene (MC4R) in food intake and body weight regulation is well characterized. MC4R mutations are the most frequent monogenic cause of human obesity. Significant associations have been revealed between MC4R mutations and productive traits in pigs, cattle and poultry. Herein, fluorescence-based conformation sensitive gel electrophoresis was used to identify two single nucleotide polymorphisms (SNPs) in the coding region (93G > A and 292G > A) and two SNPs in the 3'-UTR area (1016G > A and 1240T > C) of MC4R gene in 132 German Merino sheep. We found that the 1016G > A mutation in the 3'-UTR was significantly associated with body weight at 120 and 180 days, average daily gain, back fat thickness and loin-eye area. Allele A located at the 292th position of MC4R gene representing Arg98 was associated with significantly higher loin-eye area in sheep. For the synonymous 93G > A mutation, A allele carrier animals had higher back fat thickness. Our results provide evidence that the MC4R gene may be a candidate gene for growth and meat quality traits with MC4R SNPs being potentially valuable as genetic markers for economic traits in German Merino sheep.

• 出版日期2014-10
• 单位华中农业大学