AMP/GMP Analogs as Affinity ESIPT Probes for Highly Selective Sensing of Alkaline Phosphatase Activity in Living Systems

作者：Jia, Yan; Li, Peng; Han, Keli^*

来源：Chemistry-An Asian Journal, 2015, 10(11): 2444-2451.

DOI：10.1002/asia.201500280

• alkaline phophatase
• AMP
• fluorescence
• GMP
• selectivity

Current probes for alkaline phosphatase (ALP) detection had been developed mainly by adding a phosphate group to a dye, which would lead to indistinct performance when implemented in a living system as several phosphatases exist together. In this study, the nucleotides adenosine monophosphate (AMP) and guanosine monophosphate (GMP) were introduced into 2-(2-hydroxyphenyl)-benzothiazole-based probes, and highly fluorescent turn-on probes with good selectivity towards ALP over several phosphatases, as well as high affinity and low toxicity were obtained. In the presence of l-phenylalanine, an ALP inhibitor, a strong decrease in fluorescence recovery was observed. These probes allowed for real-time imaging of endogenous ALP activity in living cells as well as in a zebrafish model.

• 出版日期2015-11
• 单位中国科学院; 中国科学院大连化学物理研究所; 分子反应动力学国家重点实验室