A bi-layer electrospun nanofiber membrane for plasmid DNA recovery from fermentation broths

作者：Correia Tiago R; Antunes Bernardo P; Castilho Pedro H; Nunes Jose C; Pessoa de Amorim Maria T; Escobar Isabel C; Queiroz Joao A; Correia Ilidio J^*; Morao Antonio M

来源：Separation and Purification Technology, 2013, 112: 20-25.

DOI：10.1016/j.seppur.2013.03.049

• Microfiltration
• Electrospinning
• Bi-layer membrane
• Lysate
• Plasmid DNA

The demanding ever-increasing quantities of highly purified biomolecules by bio-industries, has triggered the development of new, more efficient, purification techniques. The application of membrane-based technologies has become very attractive in this field, for their high throughput capability, simplicity of operation and scale-up. %26lt;br%26gt;Herein we report the production of a bi-layer membrane by electrospinning (ES), in which a support of poly epsilon-caprolactone nanofibers was coated with a polyethylene oxide/sodium alginate layer, and subsequently cross-linked with calcium chloride. The membranes were characterized by SEM, ATR-FTIR, contact angle measurements, and were applied in the recovery process of a plasmid. The results show that membranes retained the suspended solids while allowing the permeation of plasmid DNA, with high recovery yields and improved RNA retention. Moreover, they also showed a very low fouling tendency. To the best of our knowledge it is the first time that ES membranes are applied in this type of bioprocess.

• 出版日期2013-7-10