Diamine Oxidase as a Marker for Diagnosis of Superior Mesenteric Arterial Occlusion

作者：Cai Changmao; Li Wei^*; Chen Juntian; Li Xinxin; Chen Shenglin

来源：Hepato-Gastroenterology, 2012, 59(113): 155-158.

DOI：10.5754/hge11226

• Superior mesenteric arterial occlusion (acute intestinal ischemia)
• Early diagnosis
• Diamine oxidase

Background/Aims: We investigated changes in serum diamine oxidase (DAD) activity during superior mesenteric arterial occlusion. We aimed to evaluate its value in the early diagnosis of superior mesenteric arterial occlusion. Methodology: Seventy mature male Sprague-Dawley rats were used in the study. These were divided into 7 groups of 10 rats each, : 10min, 15min, 30min, 45min, 60min and 90min superior mesenteric arterial occlusion (SMA-O) groups, and a sham group. Blood samples were taken at the indicated time points for measuring serum DAD activity. Simultaneously, the small-intestinal segments were assessed histologically and graded according to Chiu's score. Results: In the 15min group, SMA-O resulted in a rapid increase in DAD activity. Serum DAD activity and the mucosal injury score fitted well with the cubic model (r(2)=0.985, p<0.01). There was a positive correlation between ischemic duration and small-intestinal mucosal injury (r=0.909, p<0.01). Taking DAO=29.81U/L as a early diagnostic standard for superior mesenteric arterial occlusion, the sensitivity, accuracy and specificity were 94.34% (50/53), 95.71% (67/70), 100% (17/17), respectively. Conclusions: Serum DAD activity is a sensitive predictor of small-intestinal injury. Our finding suggests that measurement of serum DAD levels might provide a marker for early diagnosis of superior mesenteric arterial occlusion.

• 出版日期2012-2
• 单位汕头大学