Annexin A1 Negatively Regulates Viral RNA Replication of Hepatitis C Virus

作者：Hiramoto Hiroki; Dansako Hiromichi; Takeda Midori; Satoh Shinya; Wakita Takaji; Ikeda Masanori; Kato Nobuyuki^*

来源：Acta Medica Okayama, 2015, 69(2): 71-78.

• HCV
• annexin A1
• Li23 cell line
• Li23-derived D7 cells
• HCV-JFH-1

Persistent infection with hepatitis C virus (HCV) often causes chronic hepatitis, and then shows a high rate of progression to liver cirrhosis and hepatocellular carcinoma. To clarify the mechanism of the persistent HCV infection is considered to be important for the discovery of new target(s) for the development of anti-HCV strategies. In the present study, we found that the expression level of annexin A1 (ANXA1) in human hepatoma cell line Li3-derived D7 cells was remarkably lower than that in parental Li23 cells, whereas the susceptibility of D7 cells to HCV infection was much higher than that of Li23 cells. Therefore, we hypothesized that ANXA1 negatively regulates persistent HCV infection through the inhibition of viral RNA replication. The results revealed that HCV production was significantly inhibited without a concomitant reduction in the amount of lipid droplets in the D7 cells stably expressing exogenous ANXAL Further, we demonstrated that ANXA1 negatively regulated the step of viral RNA replication rather than that of viral entry in human hepatocytes. These results suggest that ANXA1 would be a novel target for the development of anti-HCV strategies.

• 出版日期2015-4