Anatomical and histochemical characterization of in vitro haustorium from roots of Castilleja tenuiflora

作者：Salcedo Morales G; Jimenez Aparicio A R; Cruz Sosa F; Trejo Tapia G^*

来源：Biologia Plantarum, 2014, 58(1): 164-168.

DOI：10.1007/s10535-013-0369-2

• catechin
• flavonoids
• hemiparasitism
• hydrogen peroxide
• vanillin

In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 mu M catechin, 25 mu M vanillin, or 25 mu M H2O2. Of the treatments tested, 25 mu M vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids.

• 出版日期2014-3