A new green technology for direct production of low molecular weight chitosan

作者：Wang Weiping; Du Yumin^*; Qiu Yanlin; Wang Xiaoying; Hu Ying; Yang Jianhong; Cai Jun; Kennedy John F

来源：Carbohydrate Polymers, 2008, 74(1): 127-132.

DOI：10.1016/j.carbpol.2008.01.025

• Absidia coerulea
• green technology
• low molecular weight chitosan
• solid-state fermentation
• media optimization

Low molecular weight chitosan (LMW-chitosan) in the molecular range 5-10 kDa was firstly prepared directly from the Absidia coerulea mycelia. To improve the LMW-chitosan production, the solid-state fermentation media were optimized to investigate the influence Of Substrate and Supplemental medium components on LMW-chitosan production. The LMW-chitosan was obtained after treatments with 2% NaOH and 10% acetic acid. Maximal LMW-chitosan production was 6.12 g/kg substrate. Gel permeation chromatography combined with laser light scattering gave it M-w of 6.4 kDa with M-w/M-n of 1.09. FT-IR, X-ray diffraction and C-13 NMR spectra of the product showed typical peak distributions the same as those of standard chitosan which confirmed the extracted product to be chitosan-like. The method provided a new, simple and green technology to produce LMW-chitosan directly.

• 出版日期2008-10-1
• 单位湖北工业大学; 武汉大学