A nanoliter microfluidic serial dilution bioreactor

作者：Gu Guo Yue; Lee Yi Wei; Chiang Chih Chung; Yang Ya Tang^*

来源：Biomicrofluidics, 2015, 9(4): 044126.

DOI：10.1063/1.4929946

Bacterial culture is a basic technique in both fundamental and applied microbiology. The excessive reagent consumption and laborious maintenance of bulk bioreactors for microbial culture have prompted the development of miniaturized on-chip bioreactors. With the minimal choice of two compartments (N = 2) and discrete time, periodic dilution steps, we realize a microfluidic bioreactor that mimics macroscopic serial dilution transfer culture. This device supports automated, long-term microbial cultures with a nanoliter-scale working volume and real-time monitoring of microbial populations at single-cell resolution. Because of the high surface-to-volume ratio, the device also operates as an effective biofilm-flow reactor to support cogrowth of planktonic and biofilm populations. We expect that such devices will open opportunities in many fields of microbiology.

• 出版日期2015-7
• 单位清华大学