Use of colloidal quantum dots as a digitally switched swept light source for gold nanoparticle based hyperspectral microscopy

作者：Hoshino Kazunori^*; Joshi Pratixa P; Bhave Gauri; Sokolov Konstantin V; Zhang Xiaojing

来源：Biomedical Optics Express, 2014, 5(5): 1610-1615.

DOI：10.1364/OE.22.001610

We propose a method to utilize colloidal quantum dots (QDs) as a swept light source for hyperspectral microscopy. The use of QD allows for uniform multicolor emission which covers visible-NIR wavelengths. We used 8 colors of CdSe/ZnS and CdTe/ZnS colloidal quantum dots with the peak emission wavelengths from 520 nm to 800 nm. The QDs are packed in a compact enclosure, composing a low-cost, solid-state swept light source that can be easily used in most microscopes. Multicolor emission from the QDs is simply controlled by digitally switching excitation UVLEDs, eliminating the use of mechanically-driven gratings or filters. We used gold nanoparticles as optical markers for hyperspectral microscopy. Due to the effect of localized surface plasmon resonance, gold nanoparticles demonstrate size and shape-dependent absorption spectra. Employed in a standard microscope, the QD light source enabled multispectral absorption imaging of macrophage cells labeled with gold nanorods and nanospheres.

• 出版日期2014-5-1