Anti-histone acetyltransferase activity from allspice extracts inhibits androgen receptor-dependent prostate cancer cell growth

作者：Lee Yoo Hyun; Hong Soon Won; Jun Woojin; Cho Hong Yon; Kim Han Cheon; Jung Myung Gu; Wong Jiemin; Kim Ha Il; Kim Chang Hoon; Yoon Ho Geun^*

来源：Bioscience Biotechnology and Biochemistry, 2007, 71(11): 2712-2719.

DOI：10.1271/bbb.70306

• allspice
• histone acetyltransferase
• androgen receptor
• acetylation
• prostate cancer

Histone acetylation depends on the activity of two enzyme families, histone acetyltransferase (HAT) and deacetylase (HDAC). In this study, we screened various plant extracts to find potent HAT inhibitors. Hot water extracts of allspice inhibited HAT activity, especially p300 and CBP (40% at 100 mu g/ml). The mRNA levels of two androgen receptor (AR) regulated genes, PSA and TSC22, decreased with allspice treatment (100 mu g/ml). Importantly, in IP western analysis, AR acetylation was dramatically decreased by allspice treatment.
Furthermore, chromatin immunoprecipitation indicated that the acetylation of histone H3 in the PSA and B2M promoter regions was also repressed. Finally, allspice treatment reduced the growth of human prostate cancer cells, LNCaP (50% growth inhibition at 200 mu g/ml). Taken together, our data indicate that the potent HAT inhibitory activity of allspice reduced AR and histone acetylation and led to decreased transcription of AR target genes, resulting in inhibition of prostate cancer cell growth.

• 出版日期2007-11
• 单位华东师范大学