A solid-phase extraction procedure for the clean-up of thiram from aqueous solutions containing high concentrations of humic substances

作者：Filipe O M S; Vidal M M; Duarte A C; Santos E B H^*

来源：Talanta, 2007, 72(3): 1235-1238.

DOI：10.1016/j.talanta.2007.01.005

• thiram
• solid-phase extraction
• humic substances
• aqueous samples
• UV-vis spectrophotometry

A simple solid-phase extraction (SPE) procedure with an octadecyl bo from aqueous solutions containing high concentrations of humic substances, for future studies of thiram adsorption onto solid humic substances or soils. Suspensions of humic acids and soil, in aqueous 0.01 M CaCl2 solution, were prepared and used as samples. These extracts were spiked with thiram and immediately applied to a C-18-SPE cartridge. Thiram, was eluted with chloroform and its concentration measured by spectrophotometry at 283 nm. Non-spiked aqueous extracts (blanks) and a control sample of thiram in 0.01 M CaCl2 aqueous solution were also prepared and submitted to the same SPE procedure. The results show that humic substances are extensively retained by the C-18 cartridge but are not eluted with CHCl3, Recoveries of 100-104% were obtained for thiram in the presence of humic substances. The SPE procedure described in this work is an efficient clean-up step to remove the interference of humic substances absorbance and to be coupled to any spectrophotometric or HPLC-UV method, usually used for thirarn analysis in food extracts.

• 出版日期2007-5-15