Anti-inflammatory Effects of Oct4/Sox2-overexpressing Human Adipose Tissue-derived Mesenchymal Stem Cells

作者：Li Qiang; Han Sei Myoung; Song Woo Jin; Park Sang Chul; Ryu Min Ok; Youn Hwa Young^*

来源：In Vivo, 2017, 31(3): 349-356.

DOI：10.21873/invivo.11066

• Inflammation
• mesenchymal stem cell
• Oct4
• Sox2
• lipopolysaccharide

Background/Aim: The transcription factors Oct4 and Sox2 enhance the proliferation and pluripotency of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs); however, the anti-inflammatory effects of Oct4-and Sox2-overexpressing hAT-MSCs (Oct4/Sox2-hAT-MSCs) are unclear. Here, we evaluated the anti-inflammatory effects of Oct4/Sox2-hAT-MSCs in vitro and in vivo. Materials and Methods: Supernatants from green-fluorescent protein (GFP)-and Oct4/Sox2-hAT-MSCs were used to treat lipopolysaccharide (LPS)-stimulated RAW264.7 cells and inflammatory cytokine expression was determined. In LPSinduced mice, GFP-and Oct4/Sox2-hAT-MSCs were injected intraperitoneally and survival rates, as well as sickness scores of mice, were monitored. Results: Decreased expression of pro-inflammatory cytokines was observed in Oct4/Sox2-hAT-MSC supernatant-exposed RAW264.7 cells compared to that in GFP-hAT-MSC supernatant-exposed RAW264.7 cells. The sickness score was reduced to 34.9% and the survival rate was increased by 11.1% in Oct4/Sox2-hAT- MSC-injected mice compared to that in GFP-hAT-MSC-injected mice. Conclusion: Our findings provide important insights into the development of therapies utilizing Oct4/Sox2-hAT-MSCs in inflammatory diseases.

• 出版日期2017-6